DNA Synthesis Next Generation

Overview

Development of next-generation DNA synthesis methods

Yannick RONDELEZ, DR CNRS

DNA synthesis is traditionally carried out by growing the polymer step by step through cycles of base coupling/deprotection. The historical approach, which uses phosphoramidite chemistry, does not seem suited to future DNA data storage needs, given the slow synthesis speed and the difficulty in creating long strands.

A more recent synthesis approach uses enzymatic coupling with polymerases. This technique is more environmentally friendly but is still slow and costly.

The main objective of the NGSD (Next Generation Synthesis of DNA) project is to develop a new generation of DNA synthesis that is faster and better suited to the constraints of digital information storage. This synthesis will be faster and more scalable. Ultimately, these technologies should enable integration into parallelised microchips by the end of the project.

Keywords : enzymatic DNA synthesis, high throughput, microfluidics, fast encoding

Missions

Our researches

Create new chemical blocks

Design and prepare specialised monomers and nucleotides for enzymatic synthesis. A facility dedicated to the production of new synthons will be created.

Assemble the chemical blocks

Creating and optimising enzymatic tools to increase the efficiency and speed of molecular coding

Miniaturization and parallelization DNA synthesis

Optimising and adapting matrix technologies and combining them with micropatterning and microfluidic techniques

Integrate this news into a comprehensive process

Integrate devices, enzymes and chemical blocks, combining them with the encoding and decoding of the “From Digital Data to DNA” project.